GSTM1 antibody (DyLight 550)

Catalog No. ABIN6719573

The Mouse Monoclonal anti-GSTM1 antibody has been validated for FACS. It is suitable to detect GSTM1 in samples from Human.

Quick Overview for GSTM1 antibody (DyLight 550) (ABIN6719573)

Target: GSTM1 (Glutathione S-Transferase mu 1 (GSTM1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GSTM1 antibody is conjugated to DyLight 550

Application: Flow Cytometry (FACS)

Clone: 11F2

Product Details anti-GSTM1 Antibody (DyLight 550)

Purpose: Anti-Human GSTM1 DyLight® 550 conjugated Antibody(monoclonal, 11F2)

Sequence: EEEKIRVDIL ,ENQTMDNHMQ LGMICYNPEF EKLK

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Human GSTM1 DyLight® 550 conjugated Antibody (monoclonal, 11F2) (ABIN5693233)-Dyl550. Tested in Flow Cytometry applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human GSTM1, which shares 70.6% and 73.5% amino acid (aa) sequence identity with mouse and rat GSTM1, respectively.

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells1. Carless, M. A., Lea, R. A., Curran, J. E., Appleyard, B., Gaffney, P., Green, A., Griffiths, L. R. TheGSTM1 null genotype confers an increased risk for solar keratosis development in an Australian Caucasian population. J. Invest. Derm. 119: 1373-1378, 2002. 2. Zhong, S., Wyllie, A. H., Barnes, D., Wolf, C. R., Spurr, N. K. Relationship between theGSTM1 genetic polymorphism and susceptibility to bladder, breast and colon cancer.Carcinogenesis 14: 1821-1824, 1993.

Comment:

Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Target Details for GSTM1

Target: GSTM1 (Glutathione S-Transferase mu 1 (GSTM1))

Alternative Name: GSTM1

Background:

Synonyms: Glutathione S-transferase Mu 1, GST HB subunit 4, GST class-mu 1, GSTM1-1, GSTM1a-1a, GSTM1b-1b, GTH4, GSTM1, GST1

Tissue Specificity: Liver (at protein level).

Background: Glutathione S-transferase Mu 1 (gene name GSTM1) is a human glutathione S-transferase. Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-transferase that belongs to the mu class. The mu class of enzymes functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The genes encoding the mu class of enzymes are organized in a gene cluster on chromosome 1p13.3 and are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of certain drugs. Null mutations of this class mu gene have been linked with an increase in a number of cancers, likely due to an increased susceptibility to environmental toxins and carcinogens. Multiple protein isoforms are encoded by transcript variants of this gene.

Molecular Weight: 39 kDa

Gene ID: 2944

UniProt: P09488

Pathways: Negative Regulation of Transporter Activity