EHD1 antibody (AA 324-516)

Catalog No. ABIN6719599

Product Details anti-EHD1 Antibody

Target: EHD1 (EH-Domain Containing 1 (EHD1))

Binding Specificity: AA 324-516

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EHD1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for EHD1 detection. Tested with WB, IHC-P, Direct ELISA in Human,Mouse,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for EHD1 detection. Tested with WB, IHC-P, Direct ELISA in Human,Mouse,Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human EHD1 recombinant protein (Position: K324-E516).

Isotype: IgG

Application Details

Application Notes:

Application details: Western blot|0.1-0.5 μg/mL Immunohistochemistry(Paraffin-embedded Section)|0.5-1 μg/mL Direct ELISA|0.1-0.5 μg/mL

Comment:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for EHD1

Target: EHD1 (EH-Domain Containing 1 (EHD1))

Alternative Name: EHD1 (EHD1 Products)

Synonyms: AA409636 antibody, Past1 antibody, RME-1 antibody, RGD1306960 antibody, cb862 antibody, ehd1 antibody, ehd3 antibody, ehd3l antibody, wu:fb95h05 antibody, zgc:92331 antibody, MGC130676 antibody, Testilin antibody, H-PAST antibody, HPAST1 antibody, PAST antibody, PAST1 antibody, zgc:77049 antibody, EH-domain containing 1 antibody, EH-domain containing 1b antibody, EH domain containing 1 L homeolog antibody, EH domain-containing protein 1 antibody, EH domain containing 1 antibody, EH-domain containing 1a antibody, Ehd1 antibody, ehd1b antibody, ehd1.L antibody, EHD1 antibody, LOC585665 antibody, LOC100091823 antibody, ehd1a antibody

Background:

Synonyms: EH domain-containing protein 1, PAST homolog 1, hPAST1, Testilin, EHD1, PAST, CDABP0131

Background: EH domain-containing protein 1, also known as testilin or PAST homolog 1 (PAST1), is a protein that in humans is encoded by the EHD1 gene, belonging to the EHD protein family. It is mapped to 11q13.1. This gene belongs to a highly conserved gene family encoding EPS15 homology (EH) domain-containing proteins. The protein-binding EH domain was first noted in EPS15, a substrate for the epidermal growth factor receptor. The EH domain has been shown to be an important motif in proteins involved in protein-protein interactions and in intracellular sorting. The protein encoded by this gene is thought to play a role in the endocytosis of IGF1 receptors. Alternatively spliced transcript variants have been found for this gene.

Gene ID: 10938

UniProt: Q9H4M9

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development