BAT3 antibody

Catalog No. ABIN6719601

Product Details anti-BAT3 Antibody

Target: BAT3 (Large Proline-Rich Protein BAT3 (BAT3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BAT3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)

Purpose: Rabbit IgG polyclonal antibody for BAT3/BAG6 detection. Tested with WB, IHC-P, IHC-F, ICC, FCM, Direct ELISA in Human,Mouse,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for BAT3/BAG6 detection. Tested with WB, IHC-P, IHC-F, ICC, FCM, Direct ELISA in Human,Mouse,Rat.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human BAT3/BAG6 recombinant protein (D15-A88).

Isotype: IgG

Application Details

Application Notes:

Application details: Western blot|0.1-0.5 μg/mL Immunohistochemistry(Paraffin-embedded Section)|0.5-1 μg/mL Immunohistochemistry(Frozen Section)|0.5-1 μg/mL Immunocytochemistry|0.5-1 μg/mL Flow Cytometry|1-3 μg/1x106 cells Direct ELISA|0.1-0.5 μg/mL

Comment:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for BAT3

Target: BAT3 (Large Proline-Rich Protein BAT3 (BAT3))

Alternative Name: BAG6 (BAT3 Products)

Synonyms: ARABIDOPSIS THALIANA BCL-2-ASSOCIATED ATHANOGENE 6 antibody, ATBAG6 antibody, BCL-2-associated athanogene 6 antibody, T3F17.11 antibody, 2410045D21Rik antibody, AA408914 antibody, BAG-6 antibody, Bat3 antibody, D17H6S52E antibody, G3 antibody, Scythe antibody, BAT3 antibody, D6S52E antibody, BCL-2-associated athanogene 6 antibody, BCL2-associated athanogene 6 antibody, BCL2 associated athanogene 6 antibody, BAG6 antibody, Bag6 antibody

Background:

Synonyms: Large proline-rich protein BAG6, BAG family molecular chaperone regulator 6, BCL2-associated athanogene 6, BAG-6, HLA-B-associated transcript 3, Protein G3, Protein Scythe, BAG6, BAT3

Background: Large proline-rich protein BAT3 is a protein that in humans is encoded by the BAT3 gene. It is mapped to 6p21.33. This gene was first characterized as part of a cluster of genes located within the human major histocompatibility complex class III region. This gene encodes a nuclear protein that is cleaved by caspase 3 and is implicated in the control of apoptosis. In addition, the protein forms a complex with E1A binding protein p300 and is required for the acetylation of p53 in response to DNA damage. Multiple transcript variants encoding different isoforms have been found for this gene.

Gene ID: 7917

UniProt: P46379