NOX5 antibody (AA 13-197)

Catalog No. ABIN6719638

Product Details anti-NOX5 Antibody

Target: NOX5 (NADPH Oxidase 5 (NOX5))

Binding Specificity: AA 13-197

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NOX5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Purpose: Rabbit IgG polyclonal antibody for NOX5 detection. Tested with WB, FCM, Direct ELISA in Human.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for NOX5 detection. Tested with WB, FCM, Direct ELISA in Human.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human NOX5 recombinant protein (Position: E13-F197).

Isotype: IgG

Application Details

Application Notes:

Application details: Western blot|0.1-0.5 μg/mL Flow Cytometry|1-3 μg/1x106 cells Direct ELISA|0.1-0.5 μg/mL

Comment:

Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for NOX5

Target: NOX5 (NADPH Oxidase 5 (NOX5))

Alternative Name: NOX5 (NOX5 Products)

Synonyms: NADPH oxidase 5 antibody, NOX5 antibody

Background:

Synonyms: NADPH oxidase 5, NOX5

Background: NOX5(Nadph Oxidase 5), also known as NOX5A or NOX5B, is a protein which in humans is encoded by the NOX5 gene. The International Radiation Hybrid Mapping Consortium mapped the NOX5 gene to chromosome 15. NOX5 is a novel NADPH oxidase that generates superoxide and functions as an H+ channel in a Ca2+-dependent manner. It is found that, when heterologously expressed, NOX5 was quiescent in unstimulated cells. However, in response to elevations of the cytosolic Ca2+ concentration, it generated large amounts of superoxide. Using RT-PCR and Southern and Western blot analyses, NOX5 was identified as a flavin-containing Ca2+-dependent oxidase present in hairy leukemic cells (HC), but not normal B cells.

Pathways: Proton Transport