Fluorescein antibody (DyLight 649)

Catalog No. ABIN6720647

Supplier Product No.: 600-143-096

The Goat Polyclonal anti-Fluorescein antibody is suitable to detect Fluorescein. It has been validated for WB, FLISA, FM and DB.

Quick Overview for Fluorescein antibody (DyLight 649) (ABIN6720647)

Target: Fluorescein

Host: Goat

Clonality: Polyclonal

Conjugate: DyLight 649

Application: Western Blotting (WB), FLISA, Fluorescence Microscopy (FM), Dot Blot (DB)

Product Details

Supplier Product No.: 600-143-096

Supplier: Rockland

Purpose: Fluorescein Antibody Dylight™ 649 Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and Fluorescein conjugated Bovine Serum Albumin.

Purification: Fluorescein Antibody Dylight™ 649 Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogen: Fluorescein conjugated to Goat IgG

Isotype: IgG

Labeling Ratio: 3.7

Application Details

Application Notes:

FLISA_Dilution: >1:10,000

IF_Microscopy_Dilution: >1:5,000

Other: User Optimized

Comment:

Fluorescein Antibody Dylight™ 649 Conjugated has been tested by dot blot and western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 100 μL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details

Target: Fluorescein

Target Type: Chemical

Background:

Synonyms: goat Anti-FITC antibody DyLight™ 649 conjugation, DyLight™ 649 conjugated goat anti-fluorescein antibody, DyLight™ 649 conjugated goat Anti-FITC antibody, goat anti-fluorescein antibody DyLight™ 649 conjugation

Background: Fluorescence technology is widely used to detect proteins. However, many common visible fluorophores often result in considerable background fluorescence in the visible range. Visible fluorophores are rarely used for membrane-based protein detection because of this high background. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation. Dylight™ antibody and reagent conjugates are specifically designed for protein detection methods that cover much of the visible spectrum and into the near-infrared region to visualize proteins in western blotting and other applications. Very low background fluorescence in the IR range provides for a much higher signal-to-noise ratio than visible fluorophores. Detection levels in the picogram range on Western blots rival the sensitivity of chemiluminescence on film. Dylight™conjugates provide an ultra-sensitive and convenient alternative to standard chemiluminescent protein detection methods, as well as a valuable tool for multicolor imaging.