VSV-g Tag antibody (AA 501-511) (DyLight 488)

Catalog No. ABIN6720652

Supplier Product No.: 600-441-386

This Rabbit Polyclonal antibody specifically detects VSV-g Tag in WB, FLISA and FM. It has been mentioned in 2+ publications

Quick Overview for VSV-g Tag antibody (AA 501-511) (DyLight 488) (ABIN6720652)

Target: VSV-g Tag (VSV-G Epitope Tag)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VSV-g Tag antibody is conjugated to DyLight 488

Application: Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)

Product Details anti-VSV-g Tag Antibody (DyLight 488)

Binding Specificity: AA 501-511

Supplier Product No.: 600-441-386

Supplier: Rockland

Purpose: VSV-G Epitope Tag Antibody Dylight™ 488 Conjugated

Cross-Reactivity (Details): The antibody recognizes the VSV-G epitope tag (Tyr-Thr-Asp-Ile-Glu-Met-Asn-Arg-Leu-Gly-Lys) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.

Purification: This affinity purified antibody is directed against the VSV-G epitope tag and is useful in determining its presence in over expressed proteins in various assays.

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 501-511 (Y-T-D-I-E-M-N-R-L-G-K) of vesicular stomatitis virus glycoprotein (VSV-G) conjugated to KLH using maleimide.

Isotype: IgG

Application Details

Application Notes:

FLISA_Dilution: >1:20,000

IF_Microscopy_Dilution: >1:5,000

Western_Blot_Dilution: 1:10,000 - 1:25,000

Other: User Optimized

Comment:

Suggested Applications: IF, Other
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 100 μL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

References for anti-VSV-g Tag antibody (DyLight 488) (ABIN6720652)

Morri, Sanchez-Romero, Tichy, Kainrath, Gerrard, Hirschfeld, Schwarz, Janovjak: "Optical functionalization of human Class A orphan G-protein-coupled receptors." in: Nature communications, Vol. 9, Issue 1, pp. 1950, (2018) (PubMed).

Azizi, Mironov, Muharemagic, Wehbe, Bell, Berezovski: "Viral quantitative capillary electrophoresis for counting and quality control of RNA viruses." in: Analytical chemistry, Vol. 84, Issue 21, pp. 9585-91, (2013) (PubMed).

Target Details for VSV-g Tag

Target: VSV-g Tag (VSV-G Epitope Tag)

Target Type: Tag

Background:

Synonyms: Rabbit Anti-VSV-G Epitope Tag DyLight 488™ Conjugated Antibody, Rabbit Anti VSV-G Epitope Tag DyLight 488™ Conjugated Antibody, Rabbit Anti-VSV-G Tag Antibody DyLight 488™ Conjugation

Background: In order to improve expression levels, solubility, folding, purification and detection of recombinant proteins, a very common strategy is the fusion of peptides or proteins also known as ""tags"", to the target protein. Because these tags are entities with known sequences and well characterized physicochemical properties, they are an essential tool in molecular biology that facilitates expression and purification of recombinant proteins. Because fusion tags constitute themselves antigenic epitopes for which antibodies can be developed they particularly useful for specific detection of the target protein. This Anti-VSV-G Epitope Tag Antibody generated in rabbit is conjugated to DyLight™488.