GFP antibody (DyLight 488)

Catalog No. ABIN6720700

Supplier Product No.: 200-341-215

Product Details anti-GFP Antibody (DyLight 488)

Target: GFP (Green Fluorescent Protein (GFP))

Reactivity: Aequorea victoria

Host: Mouse

Clonality: Monoclonal

Conjugate: This GFP antibody is conjugated to DyLight 488

Application: Western Blotting (WB), ELISA, Fluorescence Microscopy (FM), FLISA

Supplier Product No.: 200-341-215

Supplier: Rockland

Purpose: GFP Antibody Dylight™ 488 Conjugated

Specificity: Wt, rGFP, eGFP

Cross-Reactivity (Details): Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum.

Purification: GFP Dylight™ 488 Conjugated Antibody was prepared from tissue culture supernatant by Protein A affinity chromatography.

Immunogen: BALB/c mice were immunized with a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246 aa) derived from the jellyfish Aequorea victoria.  A hybridoma was produced by the fusion of BALB/c mouse splenocytes and myeloma cells using conventional hybridoma technology.

Clone: 9F9-F9

Isotype: IgG1 kappa

Labeling Ratio: 3.2

Application Details

Application Notes:

FLISA_Dilution: >1:20,000

IF_Microscopy_Dilution: >1:5,000

Western_Blot_Dilution: 1:10,000 - 1:25,000

Other: User Optimized

Comment:

Suggested Applications: IF
Anti-GFP Dylight™ 488 Conjugated has been tested by ELISA and western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 100 μL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

References for anti-GFP antibody (DyLight 488) (ABIN6720700)

Khosravi, Schindele, Gladilin, Dunemann, Rutten, Puchta, Houben: "Application of Aptamers Improves CRISPR-Based Live Imaging of Plant Telomeres." in: Frontiers in plant science, Vol. 11, pp. 1254, (2020) (PubMed).

Target Details for GFP

Target: GFP (Green Fluorescent Protein (GFP))

Alternative Name: GFP (GFP Products)

Background:

Synonyms: mouse anti-GFP Antibody Dylight™ 488 Conjugation, Dylight™ 488 Conjugated mouse anti-GFP Antibody, GFP, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish

Background: GFP monoclonal antibody is Dylight™ 488 Conjugated. Labeled GFP Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. DyLight™ dyes are exceptionally bright and photostable and are optimized for microscopy and microarray detection methods.

UniProt: P42212