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HIF1A antibody (Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor)) (AA 351-400) Primary Antibody

HIF1A Reactivity: Human, Mouse, Rat IF (cc), IF (p), IHC (p), WB Host: Rabbit Polyclonal unconjugated
Pubmed (10)
Catalog No. ABIN672546
$374.00
Plus shipping costs $45.00
100 μL
local_shipping Shipping to: United States
Delivery in 4 to 6 Business Days
  • Target See all HIF1A Antibodies
    HIF1A
    Binding Specificity
    • 19
    • 19
    • 16
    • 15
    • 14
    • 11
    • 9
    • 7
    • 7
    • 6
    • 6
    • 5
    • 5
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 351-400
    Reactivity
    • 203
    • 94
    • 92
    • 36
    • 21
    • 16
    • 13
    • 9
    • 9
    • 8
    • 8
    • 7
    • 6
    • 6
    • 5
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Mouse, Rat
    Host
    • 169
    • 67
    • 5
    Rabbit
    Clonality
    • 174
    • 67
    Polyclonal
    Conjugate
    • 127
    • 29
    • 17
    • 9
    • 6
    • 4
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This HIF1A antibody is un-conjugated
    Application
    • 171
    • 95
    • 77
    • 65
    • 49
    • 37
    • 35
    • 29
    • 25
    • 25
    • 12
    • 9
    • 7
    • 6
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
    Purification
    Purified by Protein A.
    Immunogen
    KLH conjugated synthetic peptide derived from human of human HIF-1 Alpha
    Isotype
    IgG
  • Application Notes
    WB 1:100-1000
    IHC-P 1:100-500
    IF(IHC-P) 1:50-200
    IF(ICC) 1:50-200
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 μg/μL
    Buffer
    Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store at -20°C
    Expiry Date
    12 months
  • Talwar, Bauerfeld, Bouhamdan, Farshi, Liu, Samavati: "MKP-1 negatively regulates LPS-mediated IL-1β production through p38 activation and HIF-1α expression." in: Cellular signalling, Vol. 34, pp. 1-10, 2017 (PubMed). (Sample species: Mouse (Murine)).

    Talwar, Bauerfeld, Liu, Samavati: "The dataset describes: HIF-1 α expression and LPS mediated cytokine production in MKP-1 deficient bone marrow derived murine macrophages." in: Data in brief, Vol. 14, pp. 56-61, 2017 (PubMed). Method employed by authors: Western Blotting (WB)

    Madka, Mohammed, Li, Zhang, Biddick, Patlolla, Lightfoot, Towner, Wu, Steele, Kopelovich, Rao: "Targeting mTOR and p53 Signaling Inhibits Muscle Invasive Bladder Cancer In Vivo." in: Cancer prevention research (Philadelphia, Pa.), Vol. 9, Issue 1, pp. 53-62, 2016 (PubMed). Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Mouse (Murine)). Further details: Sample: urothelial tumors

    Yang, Lu, Guo: "Platelet-rich plasma protects rat chondrocytes from interleukin-1β-induced apoptosis." in: Molecular medicine reports, Vol. 14, Issue 5, pp. 4075-4082, 2016 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Rat (Rattus)). Further details: Sample: chondrocytes

    Zhang, Ren, Tang, Wang, Liu, Zhang, Li, Liu, Zhao, He: "Vascular normalization induced by sinomenine hydrochloride results in suppressed mammary tumor growth and metastasis." in: Scientific reports, Vol. 5, pp. 8888, 2015 (PubMed). Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Mouse (Murine)). Further details: Sample: Lung

    Woolf, Curley, Liu, Turner, Charlton, Preul, Scheck: "The Ketogenic Diet Alters the Hypoxic Response and Affects Expression of Proteins Associated with Angiogenesis, Invasive Potential and Vascular Permeability in a Mouse Glioma Model." in: PLoS ONE, Vol. 10, Issue 6, pp. e0130357, 2015 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)).

    Yang, Li, Yan, Zhang, Sun, Qu, Wang, Duan: "Expression of RAP1B is associated with poor prognosis and promotes an aggressive phenotype in gastric cancer." in: Oncology reports, Vol. 34, Issue 5, pp. 2385-94, 2015 (PubMed). Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Human). Further details: Sample: gastric cancer

    Fan, Xu, Li, Tie, Pan, Li: "Opposite angiogenic outcome of curcumin against ischemia and Lewis lung cancer models: in silico, in vitro and in vivo studies." in: Biochimica et biophysica acta, Vol. 1842, Issue 9, pp. 1742-54, 2014 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)).

    Guo, Feng, Yao, Sun, Zhu: "Transplantation of endothelial progenitor cells in treating rats with IgA nephropathy." in: BMC nephrology, Vol. 15, pp. 110, 2014 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Rat (Rattus)). Further details: Sample: Kidney

    Shou, Lin, Liang, Li, Chen: "Expression and prognosis of FOXO3a and HIF-1? in nasopharyngeal carcinoma." in: Journal of cancer research and clinical oncology, Vol. 138, Issue 4, pp. 585-93, 2012 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Human). Further details: Sample: Nasopharyngeal carcinoma

  • Target
    HIF1A
    Alternative Name
    HIF-1 alpha (HIF1A Products)
    Synonyms
    hif1a, AA959795, HIF1alpha, MOP1, bHLHe78, HIF-1A, HIF-1alpha, HIF1, HIF1-ALPHA, PASD8, HIF1-alpha, hif-1a, hypoxia inducible factor 1 alpha, hypoxia inducible factor 1, alpha subunit, hypoxia inducible factor 1 alpha subunit, HIF (Hypoxia Inducible Factor) homologa, hif-1a, Hif1a, HIF1A, hif1a, hif-1
    Background

    Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 4 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBPB and EP3. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia.

    Subcellular location: Cytoplasm, Nucleus

    Synonyms: HIF1, MOP1, PASD8, HIF-1A, bHLHe78, HIF-1alpha, HIF1-ALPHA, Hypoxia-inducible factor 1-alpha, HIF-1-alpha, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, Class E basic helix-loop-helix protein 78, Member of PAS protein 1, PAS domain-containing protein 8, HIF1A

    Gene ID
    3091
    UniProt
    Q16665
    Pathways
    Positive Regulation of Peptide Hormone Secretion, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Transition Metal Ion Homeostasis, Tube Formation, Regulation of Carbohydrate Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals
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