RANKL antibody (AA 210-317)

Catalog No. ABIN672696

This anti-RANKL antibody is a Rabbit Polyclonal antibody detecting RANKL in WB, ELISA, FACS, IF (cc), IF (p), IHC (p) and IHC (fro). Suitable for Human, Mouse, Rat and Rabbit. This Primary Antibody has been cited in 15+ publications.

Quick Overview for RANKL antibody (AA 210-317) (ABIN672696)

Target: RANKL (TNFSF11) (Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11))

Reactivity: Human, Mouse, Rat, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RANKL antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-RANKL Antibody

Binding Specificity: AA 210-317

Cross-Reactivity: Human, Mouse, Rabbit, Rat

Predicted Reactivity: Dog

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human RANKL

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-RANKL antibody (ABIN672696)

Zhan, Liu, Wang: "The Role of MicroRNA-143-5p in the Differentiation of Dental Pulp Stem Cells into Odontoblasts by Targeting Runx2 via the OPG/RANKL Signaling Pathway." in: Journal of cellular biochemistry, Vol. 119, Issue 1, pp. 536-546, (2018) (PubMed).

Dolci, Portela, Onofre de Souza, Medeiros Fossati: "Atorvastatin-induced osteoclast inhibition reduces orthodontic relapse." in: American journal of orthodontics and dentofacial orthopedics : official publication of the American Association of Orthodontists, its constituent societies, and the American Board of Orthodontics, Vol. 151, Issue 3, pp. 528-538, (2017) (PubMed).

Yu, Gong, Lin, Wang, Liu, Li: "Denervation effectively aggravates rat experimental periodontitis." in: Journal of periodontal research, Vol. 52, Issue 6, pp. 1011-1020, (2017) (PubMed).

Chen, Fu, Ma, Wu, Li, Li, Shen, Wang: "Intermittent administration of parathyroid hormone ameliorated alveolar bone loss in experimental periodontitis in streptozotocin-induced diabetic rats." in: Archives of oral biology, Vol. 83, pp. 76-84, (2017) (PubMed).

Chen, Xue, Shen, Mu, Fu: "Curcumin alleviates glucocorticoid-induced osteoporosis through the regulation of the Wnt signaling pathway." in: International journal of molecular medicine, Vol. 37, Issue 2, pp. 329-38, (2016) (PubMed).

He, Wang, Wang, Tian, Jiang, Ren, Qiu, Fu: "Effect of glucocorticoids on osteoclast function in a mouse model of bone necrosis." in: Molecular medicine reports, Vol. 14, Issue 2, pp. 1054-60, (2016) (PubMed).

Zhang, Chen, Wei, Liu, Lei, Bai: "Ginsenosides Rg3 attenuates glucocorticoid-induced osteoporosis through regulating BMP-2/BMPR1A/Runx2 signaling pathway." in: Chemico-biological interactions, Vol. 256, pp. 188-97, (2016) (PubMed).

Xiao, Hu, Li, Li: "Bone fracture healing is delayed in splenectomic rats." in: Life sciences, Vol. 173, pp. 55-61, (2016) (PubMed).

Zhang, Liu, Li, Bai: "Mycobacterium tuberculosis 10-kDa co-chaperonin regulates the expression levels of receptor activator of nuclear factor-?B ligand and osteoprotegerin in human osteoblasts." in: Experimental and therapeutic medicine, Vol. 9, Issue 3, pp. 919-924, (2015) (PubMed).

Ota, Urakawa, Kozawa, Ikuta, Hamada, Tsukushi, Shimoyama, Ishiguro, Nishida: "Expression of colony-stimulating factor 1 is associated with occurrence of osteochondral change in pigmented villonodular synovitis." in: Tumour biology, Vol. 36, Issue 7, pp. 5361-7, (2015) (PubMed).

Yu, Lv, Zhang, Zhang, Xiao, Li: "Expression of neuropeptides and bone remodeling-related factors during periodontal tissue regeneration in denervated rats." in: Journal of molecular histology, Vol. 46, Issue 2, pp. 195-203, (2015) (PubMed).

Li, Wu, Ma, Hao, Chen, Fu, Chen, Wang: "Oral administration of 5-hydroxytryptophan aggravated periodontitis-induced alveolar bone loss in rats." in: Archives of oral biology, Vol. 60, Issue 5, pp. 789-98, (2015) (PubMed).

Sun, Wang, Fu, Wang, Bi: "A Crucial Role of IL-17 in Bone Resorption During Rejection of Fresh Bone Xenotransplantation in Rats." in: Cell biochemistry and biophysics, (2014) (PubMed).

Yang, Li, Yang, Shen, Zou, Zhu, Zhang, Yang, Li: "Efficacy and safety of echinacoside in a rat osteopenia model." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2013, pp. 926928, (2013) (PubMed).

Lescarbeau, Seib, Prewitz, Werner, Kaplan: "In vitro model of metastasis to bone marrow mediates prostate cancer castration resistant growth through paracrine and extracellular matrix factors." in: PLoS ONE, Vol. 7, Issue 8, pp. e40372, (2012) (PubMed).

Target Details for RANKL

Target: RANKL (TNFSF11) (Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11))

Alternative Name: RANKL

Background:

Synonyms: ODF, OPGL, sOdf, CD254, OPTB2, RANKL, TRANCE, hRANKL2, Tumor necrosis factor ligand superfamily member 11, Osteoclast differentiation factor, Osteoprotegerin ligand, Receptor activator of nuclear factor kappa-B ligand, TNF-related activation-induced cytokine, TNFSF11

Background: Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy.

Gene ID: 8600

UniProt: O14788

Pathways: NF-kappaB Signaling