PD-L1 antibody (APC)

Catalog No. ABIN6727419

The Rat Monoclonal anti-PD-L1 antibody has been validated for FACS. It is suitable to detect PD-L1 in samples from Mouse.

Quick Overview for PD-L1 antibody (APC) (ABIN6727419)

Target: PD-L1 (CD274 (PD-L1))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This PD-L1 antibody is conjugated to APC

Application: Flow Cytometry (FACS)

Clone: 10F-9G2

Product Details anti-PD-L1 Antibody (APC)

Isotype: IgG2b, kappa

Application Details

Application Notes: Check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/10^6 cells in 100 μL volume].

Comment:

Matching Isotype Control: APC Rat IgG2b, κ Isotype Control (ABIN7469860)

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: PBS with 0.05 % Proclin300, 1 % BSA

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2~8°C and protected from prolonged exposure to light. Do not freeze.

Target Details for PD-L1

Target: PD-L1 (CD274 (PD-L1))

Alternative Name: CD274/PD-L1

Background: B7-H1, PD-L1,Programmed cell death ligand 1,B7 homolog 1,B7-H, B7H1, PDL1, PDCD1L1, PDCD1LG1,CD274, also known as B7-H1 or programmed death ligand 1 (PD-L1), is a 40 kD type I transmembrane protein and a member of the B7 family within the immunoglobulin receptor superfamily. It is expressed on T cells, B cells, NK cells, dendritic cells, IFN-γ activated endothelial cells, and monocytes. B7-H1 is one of the ligands of PD-1. The interaction of B7-H1 with PD-1 plays an important role in the inhibition of T cell responses. Other studies have shown that B7-H1 is able to costimulate T cell growth and cytokine production. CD274 is involved in costimulation essential for T cell proliferation and production of IL-10 and IFN-γ, in an IL-2-dependent and a PD-1-independent manner. Its interaction with PD-1 inhibits T cell proliferation and cytokine production.

Gene ID: 60533

UniProt: Q9EP73

Pathways: Cancer Immune Checkpoints