Fascin antibody (pSer39)

Catalog No. ABIN673011

Cited in 3+ publications. The Rabbit Polyclonal anti-Fascin antibody (ABIN673011) specifically detects Fascin in WB, FACS, ELISA, IHC (p), IF (cc), IF (p) and IHC (fro). The antibody is reactive with Human and Mouse samples.

Quick Overview for Fascin antibody (pSer39) (ABIN673011)

Target: Fascin (FSCN1)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Fascin antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Fascin Antibody

Binding Specificity: pSer39

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Rat,Dog,Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human FSCN1 around the phosphorylation site of Ser39

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Fascin antibody (ABIN673011)

Zhang, Zhao, Zhang, Hao, Yu, Min, Li, Ma, Chen, Yi, Tang, Meng, Liu, Wang, Shen, Zhang: "Decrease in male mouse fertility by hydrogen sulfide and/or ammonia can Be inheritable." in: Chemosphere, Vol. 194, pp. 147-157, (2018) (PubMed).

Król, Mucha, Majchrzak, Homa, Bulkowska, Majewska, Gajewska, Pietrzak, Perszko, Romanowska, Paw?owski, Manuali, Hellmen, Motyl: "Macrophages mediate a switch between canonical and non-canonical Wnt pathways in canine mammary tumors." in: PLoS ONE, Vol. 9, Issue 1, pp. e83995, (2014) (PubMed).

Majchrzak, Lo Re, Gajewska, Bulkowska, Homa, Paw?owski, Motyl, Murphy, Król: "Migrastatin analogues inhibit canine mammary cancer cell migration and invasion." in: PLoS ONE, Vol. 8, Issue 10, pp. e76789, (2013) (PubMed).

Target Details for Fascin

Target: Fascin (FSCN1)

Alternative Name: FSCN1

Background:

Synonyms: HSN, SNL, p55, FAN1, Fascin, 55 kDa actin-bundling protein, Singed-like protein, FSCN1

Background: Organizes filamentous actin into bundles with a minimum of 4.1:1 actin/fascin ratio. Plays a role in the organization of actin filament bundles and the formation of microspikes, membrane ruffles, and stress fibers. Important for the formation of a diverse set of cell protrusions, such as filopodia, and for cell motility and migration.

Gene ID: 6624

UniProt: Q16658