GLIS1 antibody

Catalog No. ABIN6731016

This Rat Monoclonal antibody specifically detects GLIS1 in WB and IF. It exhibits reactivity toward Human and Mouse.

Quick Overview for GLIS1 antibody (ABIN6731016)

Target: GLIS1 (GLIS Family Zinc Finger 1 (GLIS1))

Reactivity: Human, Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This GLIS1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 7-14

Product Details anti-GLIS1 Antibody

Purpose: GLIS1 antibody (mAb) (Clone 7-14)

Purification: Protein G Chromatography

Immunogen: This antibody was raised against a peptide within the amino-terminal half of human GLIS1 (GLI-similar 1), which shares homology with mouse GLIS1.

Isotype: IgG1 kappa

Application Details

Application Notes: WB*: 0.5 - 2 μg/mL IF: 1-2 μg/mL *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

Target Details for GLIS1

Target: GLIS1 (GLIS Family Zinc Finger 1 (GLIS1))

Alternative Name: GLIS1

Background: GLIS1 (Glis Family Zinc Finger 1) belongs to a GLIS subfamily of Krüppel-like zinc-finger transcription factors characterized to regulate a set of downstream targets essential for cellular functions, including pancreatic development, b-cell maturation and maintenance, and insulin production. GLIS1 is enriched in unfertilized eggs and embryos and it can be used to promote direct reprogramming of somatic cells to induced pluripotent stem cells (iPS cells). GLIS1 may regulate the expression of numerous genes, either positively or negatively, and is implicated in mitral valve development, where dysregulation may lead to mitral valve prolapse (MVP).

Molecular Weight: 95 kDa

NCBI Accession: NP_671726