Recombinant Nucleolin antibody

Catalog No. ABIN6731026

The Mouse Monoclonal anti-Nucleolin antibody has been validated for WB. It is suitable to detect Nucleolin in samples from Human and Dog.

Quick Overview for Recombinant Nucleolin antibody (ABIN6731026)

Target: Nucleolin (NCL)

Antibody Type: Recombinant Antibody

Reactivity: Human, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This Nucleolin antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Nucleolin Antibody

Brand: AbFlex®

Purification: Protein A Chromatography

Immunogen: This antibody was raised against a salt-extracted nuclear matrix preparation from MDCK (canine kidney) cells.

Isotype: IgG2a

Application Details

Application Notes:
Validated Applications:
< strong style="margin-left:50px">WB: 0.1 - 1 μg/mL

Comment:

AbFlex^® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-Tag, an avidin tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), AbFlex^® Nucleolin antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035 % sodium azide, 30 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Target Details for Nucleolin

Target: Nucleolin (NCL)

Alternative Name: Nucleolin

Molecular Weight: 100 kDa

Pathways: Ribonucleoprotein Complex Subunit Organization