CD14 antibody (AA 201-300)

Catalog No. ABIN673164

This anti-CD14 antibody is a Rabbit Polyclonal antibody detecting CD14 in WB, ELISA, ICC, IHC (p), IHC (fro), IF (cc) and IF (p). Suitable for Human, Mouse, Rat, Sheep, Dog and Rabbit. This Primary Antibody has been cited in 7+ publications.

Quick Overview for CD14 antibody (AA 201-300) (ABIN673164)

Target: CD14

Reactivity: Human, Mouse, Rat, Sheep, Dog, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD14 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-CD14 Antibody

Binding Specificity: AA 201-300

Cross-Reactivity: Dog, Human, Mouse, Rabbit, Rat, Sheep

Predicted Reactivity: Cow,Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from mouse CD14

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-CD14 antibody (ABIN673164)

Kulhankova, Kinney, Stach, Gourronc, Grumbach, Klingelhutz, Salgado-Pabón: "The Superantigen Toxic Shock Syndrome Toxin-1 Alters Human Aortic Endothelial Cell Function." in: Infection and immunity, (2018) (PubMed).

Lee, Chen, Wallace, Sung, Sheu, Chung, Chen, Lu, Ko, Sun, Chiang, Chang, Lee, Yip: "Xenogeneic human umbilical cord-derived mesenchymal stem cells reduce mortality in rats with acute respiratory distress syndrome complicated by sepsis." in: Oncotarget, Vol. 8, Issue 28, pp. 45626-45642, (2018) (PubMed).

Sun, Chen, Chang, Chiang, Sung, Chen, Chen, Chen, Kao, Chang, Lee, Yip: "Melatonin treatment enhances therapeutic effects of exosomes against acute liver ischemia-reperfusion injury." in: American journal of translational research, Vol. 9, Issue 4, pp. 1543-1560, (2017) (PubMed).

Chang, Hsu, Yang, Sung, Chen, Huang, Kao, Chen, Chen, Chiang, Yip, Lee: "Enhanced protection against renal ischemia-reperfusion injury with combined melatonin and exendin-4 in a rodent model." in: Experimental biology and medicine (Maywood, N.J.), Vol. 241, Issue 14, pp. 1588-602, (2017) (PubMed).

Nau, Seebach, Trumm, Schaible, Kontradowitz, Meier, Buechner, Marzi, Henrich: "Alteration of Masquelet's induced membrane characteristics by different kinds of antibiotic enriched bone cement in a critical size defect model in the rat's femur." in: Injury, Vol. 47, Issue 2, pp. 325-34, (2016) (PubMed).

Chen, Yu, Fan, Bian, Ma, Lu, Jin: "Sargassum fusiforme polysaccharide activates nuclear factor kappa-B (NF-?B) and induces cytokine production via Toll-like receptors." in: Carbohydrate polymers, Vol. 105, pp. 113-20, (2014) (PubMed).

Chen, Chiang, Chen, Sung, Lee, Tsai, Chang, Chen, Sun, Leu, Chang, Yang, Yip: "Melatonin treatment further improves adipose-derived mesenchymal stem cell therapy for acute interstitial cystitis in rat." in: Journal of pineal research, Vol. 57, Issue 3, pp. 248-61, (2014) (PubMed).

Target Details for CD14

Target: CD14

Alternative Name: CD14

Background:

Synonyms: Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein, CD14

Background: In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the MD-2/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules (By similarity).

Gene ID: 12475

UniProt: P10810

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades