CD59 antibody (FITC)

Catalog No. ABIN6735023

This anti-CD59 antibody is a Mouse Monoclonal antibody detecting CD59 in FACS and IF. Suitable for Human.

Quick Overview for CD59 antibody (FITC) (ABIN6735023)

Target: CD59

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD59 antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Product Details anti-CD59 Antibody (FITC)

Specificity: Binds to a 17-25kD glycoprotein, which is distributed on human cells and has a glycosyl-phosphatidylinositol (GPI) linkage to the plasma membrane. Cell reactivity: Recognizes all leucocytes, platelets, fibroblasts, erythrocytes and most cell lines. It is negative on U937 and Daudi cell lines. It is reduced on affected cells from patients with PNH with a distribution reflecting the clonal nature of the disease.

Purification: Purified

Immunogen: Human erythrocytes.

Type of Immunogen: Cells

Isotype: IgG1

Application Details

Application Notes: Approved: Flo, IF (1:25)

Usage: Studies of complement and homologous restriction of complement action of the properties of GPI linked proteins and in the characterization of cells from patients with PNH. Flow cytometry and indirect immunofluorescence: 1:25. Dilute with isotonic buffer. Use 50 μL of the appropriate dilution per 1x1 x10⁶ in 100 μL buffer. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.2 % BSA, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for CD59

Target: CD59

Alternative Name: CD59

Background: Name/Gene ID: CD59

Synonyms: CD59, 16.3A5, 1F5, 1F5 antigen, CD59 glycoprotein, EL32, EJ16, G344, HRF-20, MAC-IP, MACIF, Ly-6-like protein, MEM43, MIRL, MEM43 antigen, MIN2, MIN3, MSK21, p18-20, Protectin, MAC-inhibitory protein, MIC11, T cell-activating protein, CD59 antigen, EJ30, HRF20, Human leukocyte antigen MIC11, Lymphocytic antigen CD59/MEM43, MIN1

Gene ID: 966

UniProt: P13987

Pathways: Complement System