HCN1 antibody (AA 346-368)

Catalog No. ABIN6735304

This Rabbit Polyclonal antibody specifically detects HCN1 in WB and ELISA. It exhibits reactivity toward Human, Rat, Mouse and Rabbit.

Quick Overview for HCN1 antibody (AA 346-368) (ABIN6735304)

Target: HCN1 (Hyperpolarization Activated Cyclic Nucleotide-Gated Potassium Channel 1 (HCN1))

Reactivity: Human, Rat, Mouse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HCN1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-HCN1 Antibody

Binding Specificity: AA 346-368

Specificity: The pore region of HCN1 (HCNP15-P) is very conserved among various HCNs. HCN1: mouse, rat, human, rabbit=100 % , HCN2: mouse, rat, human (86 %), HCN3: mouse (95 %), rat (90 %), HCN4: rat, human (95 %). The HCNP15-P sequence is also conserved in insect (Helothis virescens, 78 %), Drosophila (78 %) and Sea urchin (S. purpuratus, 69 %) HCNs. Therefore, antibodies to HCNP15-P should react with most HCNs and related sequences. Antibody reactivity in various species is not known. The HCNP15-P control peptide, because of its low MW (<3kD), is not suitable for Western. It should be used for ELISA or antibody blocking experiments (use 5-10 μg control peptide per 1 μg of aff pure IgG or 1 μL antiserum) to confirm antibody specificity. This antibody may be useful in studying various HCNs and to examine the possibility of immunoblocking the pore region and impact on the functionality of HCNs proteins.

Purification: Immunoaffinity purified

Immunogen: A 23-aa peptide sequence corresponding to the pore region of human HCN1 corresponding to amino acids 346-368.

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (1:10000 - 1:100000), WB (1 - 10 μg/mL)

Usage: Suitable for use in ELISA, Western Blot. Western Blot: 1:1000-1:5000 for neat serum and 1-10 μg/mL for affinity pure using ECL. ELISA: 1:10000-1:100000 using 50-100 ng of control peptide/well.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % BSA.

Handling Advice: avoid freeze thaw cycles. Store undiluted.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Store undiluted.

Target Details for HCN1

Target: HCN1 (Hyperpolarization Activated Cyclic Nucleotide-Gated Potassium Channel 1 (HCN1))

Alternative Name: HCN1

Background: Name/Gene ID: HCN1
Subfamily: Potassium channel - cyclic nucleotide gated
Family: Ion Channel

Synonyms: HCN1, BCNG1, BCNG-1, HAC2, HAC-2

Gene ID: 348980

UniProt: O60741

Pathways: Asymmetric Protein Localization