Hepcidin antibody (C-Term)

Catalog No. ABIN6735315

The Rabbit Polyclonal anti-Hepcidin antibody has been validated for WB, IHC, ELISA and IF. It is suitable to detect Hepcidin in samples from Human.

Quick Overview for Hepcidin antibody (C-Term) (ABIN6735315)

Target: Hepcidin (HAMP) (Hepcidin Antimicrobial Peptide (HAMP))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Hepcidin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-Hepcidin Antibody

Binding Specificity: C-Term

Specificity: Recognizes all 3 processed forms of human HEPC. The human sequence is conserved in all 3 human HEPC peptides (20-, 22- and 25-aa C-terminal fragments). May react with the unprocessed larger 60aa pro-HEPC if present in the samples or tissues. Species sequence Homology: Mouse: 69 % , rat: 61 %.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide sequence consisting of 13aa within the C-terminus of mature 25aa Human HEPC (KLH coupled).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (1:100000), IF, IHC, WB (1 - 10 μg/mL)

Usage: Suitable for use in ELISA, Immunohistochemistry, Immunofluorescence and Western Blot. Western Blot: 1-10 μg/mL (ECL). ELISA: 1:100000. Using 50-100 ng control peptide/well.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.5, 0.1 % BSA.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for Hepcidin

Target: Hepcidin (HAMP) (Hepcidin Antimicrobial Peptide (HAMP))

Alternative Name: HAMP / Hepcidin

Background: Name/Gene ID: HAMP

Synonyms: HAMP, Hepcidin antimicrobial peptide, LEAP-1, LEAP1, HEPC, Hepcidin, HFE2B, PLTR, Putative liver tumor regressor

Gene ID: 57817

UniProt: P81172

Pathways: Hormone Activity, Transition Metal Ion Homeostasis