PRKAA2 antibody (C-Term)

Catalog No. ABIN6735521

This anti-PRKAA2 antibody is a Rabbit Polyclonal antibody detecting PRKAA2 in WB and ELISA. Suitable for Rat.

Quick Overview for PRKAA2 antibody (C-Term) (ABIN6735521)

Target: PRKAA2 (Protein Kinase, AMP-Activated, alpha 2 Catalytic Subunit (PRKAA2))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRKAA2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-PRKAA2 Antibody

Binding Specificity: C-Term

Specificity: Recognizes AMPK-a2. No significant sequence homology is seen with AMPK-a1 or other proteins. Species sequence Homology: Human: 85 % , Mouse: 90 %.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide consisting of 20aa sequence mapping near the C-terminus of rat AMPK-a2 (KLH coupled).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (1:10000 - 1:100000), WB (1 - 10 μg/mL)

Usage: Suitable for use in ELISA and Western Blot. Western Blot: 1-10 μg/mL (ECL). ELISA: 1:10000-1:100000 using 50-100 ng of control peptide/well.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 100 mM Tris,  pH 7.5, 0.2 % BSA, 0.1 % sodium azide, 40 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for PRKAA2

Target: PRKAA2 (Protein Kinase, AMP-Activated, alpha 2 Catalytic Subunit (PRKAA2))

Alternative Name: PRKAA2 / AMPK Alpha 2

Background: Name/Gene ID: PRKAA2
Subfamily: AMPK/SNF1
Family: Protein Kinase

Synonyms: PRKAA2, ACACA kinase, Alpha2 AMPK, AMPK alpha 2, AMPK subunit alpha-2, Acetyl-CoA carboxylase kinase, AMPK-2, AMPKa2, AMPKalpha2, Ampk alpha2, AMPK2, PRKAA, AMPK, AMPK-alpha-2 chain, HMGCR kinase

Gene ID: 5563

UniProt: P54646

Pathways: AMPK Signaling, Carbohydrate Homeostasis, Chromatin Binding, Regulation of Carbohydrate Metabolic Process, Warburg Effect