POLG antibody (AA 26-276)

Catalog No. ABIN6735602

The Mouse Monoclonal anti-POLG antibody has been validated for WB and ELISA. It is suitable to detect POLG in samples from Human and Mouse.

Quick Overview for POLG antibody (AA 26-276) (ABIN6735602)

Target: POLG (Polymerase (DNA Directed), gamma (POLG))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This POLG antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-POLG Antibody

Binding Specificity: AA 26-276

Specificity: Recognizes human POLG. No cross-reactivity is seen with DNA Pol a, b or d. Antibody cross-reactivity in various other species has not been studied.

Purification: Immunoaffinity purified

Immunogen: Purified human recombinant DNA Pol gamma aa26-276.

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA (1:5000 - 1:25000), WB (1 - 5 μg/mL)

Usage: Suitable for use in ELISA and Western Blot. Western Blot: 1-5 μg/mL. The antibody recognizes >140kD DNA Pol g from HeLa and other cells and tissues. ELISA: 1:5000-1:25000 using 50-100 ng control antigen/well.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM Tris,  pH 7.5, 0.2 % BSA, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Short term: -20°C
Long term: -20°C.

Target Details for POLG

Target: POLG (Polymerase (DNA Directed), gamma (POLG))

Alternative Name: POLG

Target Type: Viral Protein

Background: Name/Gene ID: POLG

Synonyms: POLG, DNA polymerase gamma, MIRAS, MTDPS4A, PEO, POLG1, POLGA, SANDO, PolG, catalytic subunit, PolG-alpha, SCAE, DNA polymerase subunit gamma-1, MTDPS4B

Gene ID: 5428

UniProt: P54098