POLG antibody (AA 1041-1239)

Catalog No. ABIN7441298

Product Details anti-POLG Antibody

Target: POLG (Polymerase (DNA Directed), gamma (POLG))

Binding Specificity: AA 1041-1239

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLG antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Purpose: Polyclonal Antibody to Polymerase DNA Directed Gamma 1 (POLg1)

Specificity: The antibody is a rabbit polyclonal antibody raised against POLg1. It has been selected for its ability to recognize POLg1 in immunohistochemical staining and western blotting.

Cross-Reactivity: Pig, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Polymerase DNA Directed Gamma 1 (POLg1) corresdonding to Trp1041~Pro1239

Isotype: IgG

Application Details

Application Notes:

Western blotting: 1-5 μg/mL Immunocytochemistry in formalin fixed cells: 5-20 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL Immunohistochemistry in paraffin section: 5-20 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for POLG

Target: POLG (Polymerase (DNA Directed), gamma (POLG))

Alternative Name: Polymerase DNA Directed Gamma 1 (POLG Products)

Synonyms: POLG antibody, MDP1 antibody, MIRAS antibody, MTDPS4A antibody, MTDPS4B antibody, PEO antibody, POLG1 antibody, POLGA antibody, SANDO antibody, SCAE antibody, AA409516 antibody, PolgA antibody, polymerase (DNA) gamma, catalytic subunit L homeolog antibody, DNA polymerase gamma, catalytic subunit antibody, polymerase (DNA directed), gamma antibody, polg.L antibody, Polg antibody, POLG antibody, polg antibody

Target Type: Viral Protein

Background: PEO, POLG1, POLGA, SANDO, SCAE, Mitochondrial DNA polymerase catalytic subunit