ACCN4 antibody (AA 121-170)

Catalog No. ABIN6736354

This anti-ACCN4 antibody is a Rabbit Polyclonal antibody detecting ACCN4 in WB, IHC (p) and IHC. Suitable for Human, Rat, Mouse, Dog, Cow, Guinea Pig, Horse, Rabbit, Bat and Monkey.

Quick Overview for ACCN4 antibody (AA 121-170) (ABIN6736354)

Target: ACCN4 (Amiloride-Sensitive Cation Channel 4, Pituitary (ACCN4))

Reactivity: Human, Rat, Mouse, Dog, Cow, Guinea Pig, Horse, Rabbit, Bat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ACCN4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC)

Product Details anti-ACCN4 Antibody

Binding Specificity: AA 121-170

Brand: IHC-plus™

Specificity: Human ACCN4

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Guinea pig (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa121-170 of human ASIC4 (Q96FT7-2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Baboon, Monkey, Marmoset, Mouse, Rat, Shrew, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Guinea pig, Armadillo (100%), Galago, Opossum (92%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for ACCN4

Target: ACCN4 (Amiloride-Sensitive Cation Channel 4, Pituitary (ACCN4))

Alternative Name: ACCN4 / ASIC4

Background: Name/Gene ID: ASIC4
Subfamily: Sodium channel - amiloride-sensitive
Family: Ion Channel

Synonyms: ASIC4, ACCN4, Acid-sensing ion channel 4, BNAC4, Brain sodium channel 4, Spasic

Gene ID: 55515