BRD2 antibody (AA 524-573)

Catalog No. ABIN214728

This anti-BRD2 antibody is a Rabbit Polyclonal antibody detecting BRD2 in WB, IHC and IHC (p). Suitable for Human, Mouse, Cow, Dog, Rat, Horse, Pig, Hamster and Monkey.

Quick Overview for BRD2 antibody (AA 524-573) (ABIN214728)

Target: BRD2 (Bromodomain Containing 2 (BRD2))

Reactivity: Human, Mouse, Cow, Dog, Rat, Horse, Pig, Hamster, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BRD2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-BRD2 Antibody

Binding Specificity: AA 524-573

Brand: IHC-plus™

Specificity: Human BRD2

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Pig (100%) Xenopus (92%) Zebrafish (91%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa524-573 of human BRD2 (P25440, NP_005095). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Horse, Pig, Opossum (100%), Xenopus (92%), Zebrafish (91%), Guinea pig (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for BRD2

Target: BRD2 (Bromodomain Containing 2 (BRD2))

Alternative Name: BRD2 / RING3

Background: Name/Gene ID: BRD2
Subfamily: BRD
Family: Protein Kinase

Synonyms: BRD2, Bromodomain containing 2, FSRG1, KIAA9001, NaT, RING3, RNF3, O27.1.1, Bromodomain-containing 2, D6S113E, FSH

Gene ID: 6046

NCBI Accession: NP_005095

UniProt: P25440

Pathways: Chromatin Binding, SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection