BRD4 antibody (AA 651-700)

Catalog No. ABIN214729

This Rabbit Polyclonal antibody specifically detects BRD4 in WB, IHC and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Dog, Cow, Guinea Pig, Rabbit, Horse, Pig and Bat.

Quick Overview for BRD4 antibody (AA 651-700) (ABIN214729)

Target: BRD4 (Bromodomain Containing 4 (BRD4))

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Rabbit, Horse, Pig, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BRD4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-BRD4 Antibody

Binding Specificity: AA 651-700

Brand: IHC-plus™

Specificity: Human BRD4

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa651-700 of human BRD4 (O60885-2, NP_055114). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Galago, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Platypus, Lizard (100%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for BRD4

Target: BRD4 (Bromodomain Containing 4 (BRD4))

Alternative Name: BRD4

Background: Name/Gene ID: BRD4
Subfamily: BRD
Family: Protein Kinase

Synonyms: BRD4, Bromodomain containing 4, Chromosome-associated protein, MCAP, HUNK1, Protein HUNK1, Bromodomain-containing 4, HUNKI

Gene ID: 23476

NCBI Accession: NP_055114

UniProt: O60885

Pathways: Chromatin Binding, SARS-CoV-2 Protein Interactome