BRD7 antibody (C-Term)

Catalog No. ABIN6736358

The Rabbit Polyclonal anti-BRD7 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect BRD7 in samples from Human and Dog.

Quick Overview for BRD7 antibody (C-Term) (ABIN6736358)

Target: BRD7 (Bromodomain Containing 7 (BRD7))

Reactivity: Human, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BRD7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-BRD7 Antibody

Binding Specificity: C-Term

Brand: IHC-plus™

Specificity: Human BRD7

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Guinea pig (92%) Dog (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human BRD7 (Q9NPI1, NP_037395). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon (100%), Monkey, Guinea pig (92%), Galago, Marmoset, Dog, Bat (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for BRD7

Target: BRD7 (Bromodomain Containing 7 (BRD7))

Alternative Name: BRD7

Background: Name/Gene ID: BRD7

Synonyms: BRD7, 75 kDa bromodomain protein, BP75, Bromodomain containing 7, Bromodomain-containing 7, CELTIX1, NAG4, Protein CELTIX-1

Gene ID: 29117

NCBI Accession: NP_037395

UniProt: Q9NPI1