CCL7 antibody (AA 30-79)

Catalog No. ABIN6736363

This anti-CCL7 antibody is a Rabbit Polyclonal antibody detecting CCL7 in WB, IHC and IHC (p). Suitable for Human.

Quick Overview for CCL7 antibody (AA 30-79) (ABIN6736363)

Target: CCL7 (Chemokine (C-C Motif) Ligand 7 (CCL7))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCL7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CCL7 Antibody

Binding Specificity: AA 30-79

Brand: IHC-plus™

Specificity: Human CCL7 / MCP3

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa30-79 of human CCL7 (P80098, NP_006264). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey (100%), Marmoset (92%), Horse (84%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile ddH2O

Concentration: Lot specific

Buffer: Lyophilized from PBS, 2 % sucrose.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for CCL7

Target: CCL7 (Chemokine (C-C Motif) Ligand 7 (CCL7))

Alternative Name: CCL7 / MCP-3

Background: Name/Gene ID: CCL7
Subfamily: Intercrine beta
Family: Intercrine

Synonyms: CCL7, C-C motif chemokine 7, Chemokine (C-C motif) ligand 7, FIC, MARC, MCP3, Monocyte chemotactic protein 3, SCYA7, Small-inducible cytokine A7, MCP-3, SCYA6, NC28

Gene ID: 6354

NCBI Accession: NP_006264

UniProt: P80098