CDC25B antibody (AA 56-105)

Catalog No. ABIN6736364

The Rabbit Polyclonal anti-CDC25B antibody has been validated for WB, IHC and IHC (p). It is suitable to detect CDC25B in samples from Human, Mouse, Rat, Dog, Guinea Pig, Horse, Cow and Pig.

Quick Overview for CDC25B antibody (AA 56-105) (ABIN6736364)

Target: CDC25B (Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B))

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Horse, Cow, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDC25B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CDC25B Antibody

Binding Specificity: AA 56-105

Brand: IHC-plus™

Specificity: Human CDC25B

Predicted Reactivity: Percent identity by BLAST analysis: Human, Dog, Bovine, Horse, Pig, Guinea pig (100%) Rabbit (92%) Mouse, Rat (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa56-105 of human CDC25B (P30305, NP_068659). Percent identity by BLAST analysis: Human, Gorilla, Galago, Marmoset, Elephant, Panda, Dog, Bovine, Horse, Pig, Guinea pig (100%), Monkey, Bat, Rabbit (92%), Mouse, Rat (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for CDC25B

Target: CDC25B (Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B))

Alternative Name: CDC25B

Background: Name/Gene ID: CDC25B
Subfamily: CDC25
Family: Protein Phosphatase

Synonyms: CDC25B, CDC25B phosphatase, Cell division cycle 25B, M-phase inducer phosphatase 2, CDC25HU2

Gene ID: 994

NCBI Accession: NP_068659

UniProt: P30305

Pathways: Cell Division Cycle, M Phase, Autophagy