Claudin 18 antibody (C-Term)

Catalog No. ABIN214745

The Rabbit Polyclonal anti-Claudin 18 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect Claudin 18 in samples from Human, Mouse, Rat, Cow, Dog, Horse and Monkey.

Quick Overview for Claudin 18 antibody (C-Term) (ABIN214745)

Target: Claudin 18 (CLDN18)

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Claudin 18 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Claudin 18 Antibody

Binding Specificity: C-Term

Brand: IHC-plus™

Specificity: Human CLDN18 / Claudin 18

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat (100%) Rabbit (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human CLDN18 (P56856, NP_001002026). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Bovine, Horse (100%), Elephant, Rabbit, Guinea pig (92%), Panda (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Claudin 18

Target: Claudin 18 (CLDN18)

Alternative Name: CLDN18 / Claudin 18

Background: Name/Gene ID: CLDN18
Family: Claudin

Synonyms: CLDN18, Claudin 18, Claudin-18, SFTPJ, SFTA5, Surfactant associated 5

Gene ID: 51208

NCBI Accession: NP_001002026

UniProt: P56856

Pathways: Cell-Cell Junction Organization, Hepatitis C