ESR2 antibody (AA 82-131)

Catalog No. ABIN214750

This Rabbit Polyclonal antibody specifically detects ESR2 in WB, IHC and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Pig, Cow, Rabbit and Bat.

Quick Overview for ESR2 antibody (AA 82-131) (ABIN214750)

Target: ESR2 (Estrogen Receptor 2 (ESR2))

Reactivity: Human, Mouse, Rat, Pig, Cow, Rabbit, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ESR2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ESR2 Antibody

Binding Specificity: AA 82-131

Brand: IHC-plus™

Specificity: Human ESR2 / ER-Beta

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Bovine, Pig (100%) Sheep, Dog, Guinea pig (92%) Rabbit (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa82-131 of human ESR2 (Q92731, NP_001428). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Baboon, Monkey, Mouse, Rat, Bovine, Bat, Pig (100%), Galago, Marmoset, Sheep, Panda, Dog, Guinea pig (92%), Hamster, Elephant, Cat (85%), Rabbit (84%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with sterile water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for ESR2

Target: ESR2 (Estrogen Receptor 2 (ESR2))

Alternative Name: ESR2 / ER Beta

Background: Name/Gene ID: ESR2
Subfamily: NR3 Steroid receptor
Family: NHR

Synonyms: ESR2, ESTRB, ER Beta, ERbeta, ER-BETA, ESR-BETA, ESRB, Estrogen receptor 2 (ER beta), NR3A2, Estrogen receptor beta, Estrogen receptor beta 4

Gene ID: 2100

NCBI Accession: NP_001428

UniProt: Q92731

Pathways: Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling