FOXD3 antibody (AA 257-306)

Catalog No. ABIN6736379

This Rabbit Polyclonal antibody specifically detects FOXD3 in WB, IHC and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Monkey, Cow and Dog.

Quick Overview for FOXD3 antibody (AA 257-306) (ABIN6736379)

Target: FOXD3 (Forkhead Box D3 (FOXD3))

Reactivity: Human, Mouse, Rat, Monkey, Cow, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FOXD3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-FOXD3 Antibody

Binding Specificity: AA 257-306

Brand: IHC-plus™

Specificity: Human FOXD3

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa257-306 of human FOXD3 (Q9UJU5, NP_036315). Percent identity by BLAST analysis: Human, Chimpanzee, Monkey, Mouse, Rat, Elephant, Dog, Bovine (100%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (3, 5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for FOXD3

Target: FOXD3 (Forkhead Box D3 (FOXD3))

Alternative Name: FOXD3

Background: Name/Gene ID: FOXD3
Family: Transcription factor

Synonyms: FOXD3, AIS1, Drosophila forkhead homologue, Genesis, Forkhead box protein D3, Forkhead box D3, HFH2, VAMAS2

Gene ID: 27022

NCBI Accession: NP_036315

UniProt: Q9UJU5