HDAC2 antibody (AA 419-468)

Catalog No. ABIN6736395

Product Details anti-HDAC2 Antibody

Target: HDAC2 (Histone Deacetylase 2 (HDAC2))

Binding Specificity: AA 419-468

Reactivity: Human, Mouse, Monkey, Cow, Rabbit, Chicken, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HDAC2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human HDAC2

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Bovine, Rabbit, Pig, Chicken (100%) Rat, Horse, Guinea pig (92%) Xenopus (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa419-468 of human HDAC2 (Q92769, NP_001518). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Bovine, Rabbit, Pig, Opossum, Turkey, Zebra finch, Chicken, Platypus (100%), Rat, Elephant, Horse, Guinea pig, Lizard (92%), Xenopus (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for HDAC2

Target: HDAC2 (Histone Deacetylase 2 (HDAC2))

Alternative Name: HDAC2 (HDAC2 Products)

Background: Name/Gene ID: HDAC2
Family: Histone Deacetylase

Synonyms: HDAC2, HD2, Histone deacetylase 2, RPD3, YAF1, YY1-associated factor 1

Gene ID: 3066

NCBI Accession: NP_001518

Pathways: Neurotrophin Signaling Pathway, Regulation of Muscle Cell Differentiation, Negative Regulation of intrinsic apoptotic Signaling, SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection