HDAC6 antibody (AA 69-118)

Catalog No. ABIN6736396

The Rabbit Polyclonal anti-HDAC6 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect HDAC6 in samples from Human, Mouse, Monkey, Rabbit, Horse and Bat.

Quick Overview for HDAC6 antibody (AA 69-118) (ABIN6736396)

Target: HDAC6 (Histone Deacetylase 6 (HDAC6))

Reactivity: Human, Mouse, Monkey, Rabbit, Horse, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HDAC6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-HDAC6 Antibody

Binding Specificity: AA 69-118

Brand: IHC-plus™

Specificity: Human HDAC6

Predicted Reactivity: Percent identity by BLAST analysis: Human, Rabbit, Horse (100%) Rat, Bovine (92%) Mouse (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa69-118 of human HDAC6 (Q9UBN7). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Galago, Bat, Rabbit, Horse (100%), Rat, Bovine, Guinea pig (92%), Mouse, Elephant (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for HDAC6

Target: HDAC6 (Histone Deacetylase 6 (HDAC6))

Alternative Name: HDAC6

Background: Name/Gene ID: HDAC6
Family: Histone Deacetylase

Synonyms: HDAC6, Histone deacetylase 6, HD6, KIAA0901, JM21

Gene ID: 10013

UniProt: Q9UBN7

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling