Serotonin Receptor 1A antibody (N-Term)

Catalog No. ABIN6736400

This Rabbit Polyclonal antibody specifically detects Serotonin Receptor 1A in WB, IHC and IHC (p). It exhibits reactivity toward Human.

Quick Overview for Serotonin Receptor 1A antibody (N-Term) (ABIN6736400)

Target: Serotonin Receptor 1A (HTR1A)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Serotonin Receptor 1A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Serotonin Receptor 1A Antibody

Binding Specificity: N-Term

Brand: IHC-plus™

Specificity: Human HTR1A / 5-HT1A

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human HTR1A (P08908, NP_000515). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon (100%), Chimpanzee (92%), Monkey (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), WB (0.06 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Serotonin Receptor 1A

Target: Serotonin Receptor 1A (HTR1A)

Alternative Name: HTR1A / 5-HT1A Receptor

Background: Name/Gene ID: HTR1A
Subfamily: Serotonin
Family: GPCR

Synonyms: HTR1A, 5-HT-1A, 5HT1a, 5-HT1a receptor, 5HT1ar, 5HT1A receptor, 5-HT1A, ADRBRL1, ADRB2RL1, PFMCD, Serotonin 5-HT-1a receptor, Serotonin type 1a receptor, Serotonergic type 1A receptor, G-21, Serotonin 1a receptor, Serotonin receptor 1A

Gene ID: 3350

NCBI Accession: NP_000515

UniProt: P08908

Pathways: JAK-STAT Signaling, Synaptic Membrane, Feeding Behaviour