MAPK14 antibody (C-Term)

Catalog No. ABIN6736415

This anti-MAPK14 antibody is a Rabbit Polyclonal antibody detecting MAPK14 in WB, IHC and IHC (p). Suitable for Human, Mouse, Rat, Dog, Chicken, Rabbit, Cow, Monkey, Pig, Sheep, Guinea Pig, Horse and Bat.

Quick Overview for MAPK14 antibody (C-Term) (ABIN6736415)

Target: MAPK14 (Mitogen-Activated Protein Kinase 14 (MAPK14))

Reactivity: Human, Mouse, Rat, Dog, Chicken, Rabbit, Cow, Monkey, Pig, Sheep, Guinea Pig, Horse, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAPK14 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-MAPK14 Antibody

Binding Specificity: C-Term

Brand: IHC-plus™

Specificity: Human MAPK14 / p38

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Sheep, Bovine, Rabbit, Horse, Pig, Chicken (100%) Xenopus (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human MAPK14 (Q16539, NP_001306). Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Sheep, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus (100%), Xenopus (92%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for MAPK14

Target: MAPK14 (Mitogen-Activated Protein Kinase 14 (MAPK14))

Alternative Name: MAPK14 / p38

Background: Name/Gene ID: MAPK14
Subfamily: MAPK
Family: Protein Kinase

Synonyms: MAPK14, CSAID-binding protein, CSBP2, CSPB1, CSBP1, Csaids binding protein, CSBP, EXIP, MAP kinase 14, MAP kinase p38 alpha, MAX-interacting protein 2, MAPK 14, p38 alpha, PRKM15, PRKM14, RK, SAPK2A, MAP kinase MXI2, Mxi2, p38ALPHA, p38, p38 MAP kinase, p38alpha Exip

Gene ID: 1432

NCBI Accession: NP_001306

UniProt: Q16539

Pathways: MAPK Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Hepatitis C, Toll-Like Receptors Cascades, Autophagy, Thromboxane A2 Receptor Signaling, BCR Signaling, S100 Proteins