PITX3 antibody (N-Term)

Catalog No. ABIN6736423

This anti-PITX3 antibody is a Rabbit Polyclonal antibody detecting PITX3 in WB, IHC and IHC (p). Suitable for Human, Mouse, Rat, Dog, Horse, Rabbit, Pig, Bat and Monkey.

Quick Overview for PITX3 antibody (N-Term) (ABIN6736423)

Target: PITX3 (Pituitary Homeobox 3 (PITX3))

Reactivity: Human, Mouse, Rat, Dog, Horse, Rabbit, Pig, Bat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PITX3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PITX3 Antibody

Binding Specificity: N-Term

Brand: IHC-plus™

Specificity: Human PITX3

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Rabbit, Pig (100%) Sheep, Bovine, Guinea pig (92%) Dog (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human PITX3 (O75364, NP_005020). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Bat, Rabbit, Horse, Pig (100%), Sheep, Elephant, Bovine, Guinea pig (92%), Opossum (90%), Dog (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for PITX3

Target: PITX3 (Pituitary Homeobox 3 (PITX3))

Alternative Name: PITX3 / PTX3

Background: Name/Gene ID: PITX3

Synonyms: PITX3, CTPP4, Homeobox protein PITX3, Paired-like homeodomain 3, Pituitary homeobox 3

Gene ID: 5309

NCBI Accession: NP_005020

UniProt: O75364