RAB14 antibody (C-Term)

Catalog No. ABIN6736424

This Rabbit Polyclonal antibody specifically detects RAB14 in WB, IHC and IHC (p). It exhibits reactivity toward Human, Rat, Mouse, Pig, Chicken, Monkey, Dog, Cow, Goat, Horse, Guinea Pig, Rabbit, Hamster, Xenopus laevis and Bat.

Quick Overview for RAB14 antibody (C-Term) (ABIN6736424)

Target: RAB14 (RAB14, Member RAS Oncogene Family (RAB14))

Reactivity: Human, Rat, Mouse, Pig, Chicken, Monkey, Dog, Cow, Goat, Horse, Guinea Pig, Rabbit, Hamster, Xenopus laevis, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAB14 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-RAB14 Antibody

Binding Specificity: C-Term

Brand: IHC-plus™

Specificity: Human RAB14

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Goat, Pig, Chicken, Xenopus (100%) Zebrafish (93%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human RAB14 (P61106, NP_057406). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Goat, Hamster, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Xenopus (100%), Zebrafish (93%), Salmon (87%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for RAB14

Target: RAB14 (RAB14, Member RAS Oncogene Family (RAB14))

Alternative Name: RAB14

Background: Name/Gene ID: RAB14
Subfamily: RAS oncogene
Family: Ras GTPase superfamily IPR001806

Synonyms: RAB14, F protein-binding protein 1, FBP, RAB-14, Ras-related protein Rab-14

Gene ID: 51552

NCBI Accession: NP_057406

UniProt: P61106

Pathways: Asymmetric Protein Localization, SARS-CoV-2 Protein Interactome