RUNX1 antibody (N-Term)

Catalog No. ABIN6736427

This Rabbit Polyclonal antibody specifically detects RUNX1 in WB, IHC and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Horse, Pig, Rabbit, Guinea Pig, Monkey, Chicken, Zebrafish (Danio rerio) and Bat.

Quick Overview for RUNX1 antibody (N-Term) (ABIN6736427)

Target: RUNX1 (Runt-Related Transcription Factor 1 (RUNX1))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Pig, Rabbit, Guinea Pig, Monkey, Chicken, Zebrafish (Danio rerio), Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RUNX1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-RUNX1 Antibody

Binding Specificity: N-Term

Brand: IHC-plus™

Specificity: Human RUNX1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Chicken (100%) Xenopus (92%) Zebrafish (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human RUNX1 (Q01196). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig, Chicken (100%), Opossum, Zebra finch, Xenopus (92%), Zebrafish (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for RUNX1

Target: RUNX1 (Runt-Related Transcription Factor 1 (RUNX1))

Alternative Name: AML1 / RUNX1

Background: Name/Gene ID: RUNX1

Synonyms: RUNX1, AMLCR1, Aml1 oncogene, AML1, AML1-EVI-1, CBF-alpha-2, CBFA2, Acute myeloid leukemia 1, EVI-1, PEBP2A2, PEA2-alpha B, AML1-EVI-1 fusion protein, Oncogene AML-1, PEBP2-alpha B, PEBP2aB

Gene ID: 861

UniProt: Q01196