TRIM14 antibody (AA 116-165)

Catalog No. ABIN6736436

This Rabbit Polyclonal antibody specifically detects TRIM14 in WB, IHC and IHC (p). It exhibits reactivity toward Human, Dog, Rabbit and Bat.

Quick Overview for TRIM14 antibody (AA 116-165) (ABIN6736436)

Target: TRIM14 (Tripartite Motif Containing 14 (TRIM14))

Reactivity: Human, Dog, Rabbit, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRIM14 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-TRIM14 Antibody

Binding Specificity: AA 116-165

Brand: IHC-plus™

Specificity: Human TRIM14

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Mouse, Rat, Bovine, Horse, Pig (92%) Dog, Rabbit (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa116-165 of human TRIM14 (Q5TBQ8). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Bat (100%), Monkey, Marmoset, Mouse, Rat, Bovine, Horse, Pig (92%), Galago, Dog, Rabbit, Guinea pig (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for TRIM14

Target: TRIM14 (Tripartite Motif Containing 14 (TRIM14))

Alternative Name: TRIM14

Background: Name/Gene ID: TRIM14
Family: Tripartite Motif

Synonyms: TRIM14, KIAA0129, Tripartite motif containing 14, Tripartite motif-containing 14

Gene ID: 9830

UniProt: Q14142