TRIM32 antibody (AA 549-598)

Catalog No. ABIN6736439

This Rabbit Polyclonal antibody specifically detects TRIM32 in WB, IHC and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Guinea Pig, Zebrafish (Danio rerio), Pig, Chicken, Monkey and Xenopus laevis.

Quick Overview for TRIM32 antibody (AA 549-598) (ABIN6736439)

Target: TRIM32 (Tripartite Motif Containing 32 (TRIM32))

Reactivity: Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Guinea Pig, Zebrafish (Danio rerio), Pig, Chicken, Monkey, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRIM32 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-TRIM32 Antibody

Binding Specificity: AA 549-598

Brand: IHC-plus™

Specificity: Human TRIM32

Predicted Reactivity: Percent identity by BLAST analysis: Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig, Chicken (100%) Zebrafish (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa549-598 of human TRIM32 (Q13049, NP_036342). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Xenopus (100%), Stickleback, Zebrafish (84%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for TRIM32

Target: TRIM32 (Tripartite Motif Containing 32 (TRIM32))

Alternative Name: HT2A / TRIM32

Background: Name/Gene ID: TRIM32
Family: Tripartite Motif

Synonyms: TRIM32, 72 kDa Tat-interacting protein, HT2A, LGMD2H, TATIP, Tripartite motif containing 32, Zinc finger protein HT2A, Zinc-finger protein HT2A, TAT-interactive protein, 72-KD, BBS11, Tripartite motif-containing 32

Gene ID: 22954

NCBI Accession: NP_036342

UniProt: Q13049

Pathways: Negative Regulation of intrinsic apoptotic Signaling