AFT1 antibody (N-Term)

Catalog No. ABIN6736702

This anti-AFT1 antibody is a Rabbit Polyclonal antibody detecting AFT1 in WB, IHC and IHC (p). Suitable for Human, Mouse, Rat, Cow, Dog, Horse, Rabbit, Guinea Pig, Monkey and Hamster.

Quick Overview for AFT1 antibody (N-Term) (ABIN6736702)

Target: AFT1 (Activating Transcription Factor 1 (AFT1))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Rabbit, Guinea Pig, Monkey, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AFT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-AFT1 Antibody

Binding Specificity: N-Term

Brand: IHC-plus™

Specificity: Human ATF1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit (100%) Xenopus (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human ATF1 (P18846, NP_005162). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Rabbit, Horse, Guinea pig (100%), Opossum (91%), Lizard, Xenopus (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for AFT1

Target: AFT1 (Activating Transcription Factor 1 (AFT1))

Alternative Name: ATF1

Background: Name/Gene ID: ATF1
Family: Transcription factor

Synonyms: ATF1, ATF-1, EWS-ATF1, FUS/ATF-1, Protein TREB36, TREB36

Gene ID: 466

NCBI Accession: NP_005162

UniProt: P18846

Pathways: Neurotrophin Signaling Pathway, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Toll-Like Receptors Cascades