Caveolin 3 antibody (N-Term)

Catalog No. ABIN6736704

The Rabbit Polyclonal anti-Caveolin 3 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect Caveolin 3 in samples from Human, Mouse, Rat, Cow, Rabbit, Horse and Monkey.

Quick Overview for Caveolin 3 antibody (N-Term) (ABIN6736704)

Target: Caveolin 3 (CAV3)

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Horse, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caveolin 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Caveolin 3 Antibody

Binding Specificity: N-Term

Brand: IHC-plus™

Specificity: Human CAV3 / Caveolin 3

Predicted Reactivity: Percent identity by BLAST analysis: Human, Bovine (100%) Pig (92%) Mouse, Rat (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human CAV3 (P56539, NP_001225). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Panda, Bovine, Rabbit, Horse (100%), Bat, Pig, Guinea pig (92%), Elephant (86%), Galago, Mouse, Rat (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for Caveolin 3

Target: Caveolin 3 (CAV3)

Alternative Name: CAV3 / Caveolin 3

Background: Name/Gene ID: CAV3

Synonyms: CAV3, Caveolin-3, Caveolin3, Caveolin 3, LGMD1C, VIP-21, VIP21, LQT9, M-caveolin

Gene ID: 859

NCBI Accession: NP_001225

UniProt: P56539

Pathways: Carbohydrate Homeostasis, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Negative Regulation of Transporter Activity