DOK2 antibody (C-Term)

Catalog No. ABIN6736711

This Rabbit Polyclonal antibody specifically detects DOK2 in WB, IHC and IHC (p). It exhibits reactivity toward Human, Cow, Pig and Guinea Pig.

Quick Overview for DOK2 antibody (C-Term) (ABIN6736711)

Target: DOK2

Reactivity: Human, Cow, Pig, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DOK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-DOK2 Antibody

Binding Specificity: C-Term

Brand: IHC-plus™

Specificity: Human DOK2

Predicted Reactivity: Percent identity by BLAST analysis: Human, Bovine, Pig (100%) Mouse, Rat, Dog, Horse (90%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human DOK2 (O60496, NP_003965). Percent identity by BLAST analysis: Human, Gorilla, Monkey, Galago, Bovine, Pig, Guinea pig (100%), Marmoset, Mouse, Rat, Elephant, Dog, Bat, Horse (90%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for DOK2

Target: DOK2

Alternative Name: DOK2

Background: Name/Gene ID: DOK2

Synonyms: DOK2, Docking protein 2, 56kD, Dok-2, Docking protein 2, Docking protein 2, 56  kDa, p56(dok-2), p56dok-2, p56DOK

Gene ID: 9046

NCBI Accession: NP_003965

UniProt: O60496