E2F3 antibody (C-Term)

Catalog No. ABIN214098

This anti-E2F3 antibody is a Rabbit Polyclonal antibody detecting E2F3 in WB, IHC (p) and IHC. Suitable for Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Bat, Pig and Hamster.

Quick Overview for E2F3 antibody (C-Term) (ABIN214098)

Target: E2F3

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Bat, Pig, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This E2F3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC)

Product Details anti-E2F3 Antibody

Binding Specificity: C-Term

Specificity: Human E2F3

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Dog, Bovine, Pig (100%) Rabbit, Chicken (92%) Xenopus (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human E2F3 (Q68DT0). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Bat, Horse, Pig, Opossum, Guinea pig (100%), Marmoset, Rabbit, Turkey, Zebra finch, Chicken, Platypus, Lizard (92%), Panda (90%), Xenopus (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for E2F3

Target: E2F3

Background: Name/Gene ID: E2F3

Synonyms: E2F3, E2F transcription factor 3, KIAA0075, Transcription factor E2F3, E2F-3

Gene ID: 1871

UniProt: O00716