PHD1 antibody (AA 58-107)

Catalog No. ABIN6736715

The Rabbit Polyclonal anti-PHD1 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect PHD1 in samples from Human, Cow and Guinea Pig.

Quick Overview for PHD1 antibody (AA 58-107) (ABIN6736715)

Target: PHD1 (EGLN2) (Egl-9 Family Hypoxia Inducible Factor 2 (EGLN2))

Reactivity: Human, Cow, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PHD1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PHD1 Antibody

Binding Specificity: AA 58-107

Brand: IHC-plus™

Specificity: Human EGLN2 / PHD1

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Mouse, Rat, Dog, Pig (92%) Guinea pig (85%).

Purification: Protein A purified

Immunogen: Synthetic peptide located between aa58-107 of human EGLN2 (Q96KS0, NP_444274). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey (100%), Galago, Marmoset, Mouse, Rat, Dog, Pig (92%), Elephant, Guinea pig (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for PHD1

Target: PHD1 (EGLN2) (Egl-9 Family Hypoxia Inducible Factor 2 (EGLN2))

Alternative Name: EGLN2 / PHD1

Background: Name/Gene ID: EGLN2

Synonyms: EGLN2, EIT6, EGL nine (C.elegans) homolog 2, Egl nine homolog 2, HPH-1, HPH-3, Estrogen-induced tag 6, PHD1, HIF-PH1, HIF-prolyl hydroxylase 1, HIFPH1

Gene ID: 112398

NCBI Accession: NP_444274

UniProt: Q96KS0

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Cell RedoxHomeostasis