GDI2 antibody (AA 361-410)

Catalog No. ABIN6736721

This anti-GDI2 antibody is a Rabbit Polyclonal antibody detecting GDI2 in WB, IHC and IHC (p). Suitable for Human, Dog, Cow, Horse, Sheep, Chicken, Bat and Hamster.

Quick Overview for GDI2 antibody (AA 361-410) (ABIN6736721)

Target: GDI2 (GDP Dissociation Inhibitor 2 (GDI2))

Reactivity: Human, Dog, Cow, Horse, Sheep, Chicken, Bat, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GDI2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GDI2 Antibody

Binding Specificity: AA 361-410

Brand: IHC-plus™

Specificity: Human GDI2

Predicted Reactivity: Percent identity by BLAST analysis: Human, Sheep, Horse, Chicken (100%) Mouse, Pig, Guinea pig (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa361-410 of human GDI2 (P50395, NP_001485). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Galago, Marmoset, Sheep, Hamster, Elephant, Dog, Bovine, Bat, Horse, Opossum, Turkey, Chicken, Platypus (100%), Orangutan, Monkey, Mouse, Rat, Rabbit, Pig, Guinea pig, Zebra finch, Lizard, Xenopus (92%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for GDI2

Target: GDI2 (GDP Dissociation Inhibitor 2 (GDI2))

Alternative Name: GDI2

Background: Name/Gene ID: GDI2

Synonyms: GDI2, GDP dissociation inhibitor 2, GDI-2, Rab GDI beta, RABGDIB

Gene ID: 2665

NCBI Accession: NP_001485

UniProt: P50395