E2F4 antibody (C-Term)

Catalog No. ABIN6736723

The Rabbit Polyclonal anti-E2F4 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect E2F4 in samples from Human, Mouse, Rat, Cow, Horse, Rabbit, Chicken, Dog, Pig, Monkey, Guinea Pig, Zebrafish (Danio rerio) and Bat.

Quick Overview for E2F4 antibody (C-Term) (ABIN6736723)

Target: E2F4 (E2F Transcription Factor 4, P107/p130-Binding (E2F4))

Reactivity: Human, Mouse, Rat, Cow, Horse, Rabbit, Chicken, Dog, Pig, Monkey, Guinea Pig, Zebrafish (Danio rerio), Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This E2F4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-E2F4 Antibody

Binding Specificity: C-Term

Brand: IHC-plus™

Specificity: Human E2F4

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Dog, Bovine, Rabbit, Pig, Chicken, Zebrafish (100%) Xenopus (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human E2F4 (Q16254, NP_001941). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Salmon, Zebrafish (100%), Xenopus, Stickleback (92%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for E2F4

Target: E2F4 (E2F Transcription Factor 4, P107/p130-Binding (E2F4))

Alternative Name: E2F4

Background: Name/Gene ID: E2F4

Synonyms: E2F4, E2F-4, p107/p130-binding protein, Transcription factor E2F4

Gene ID: 1874

NCBI Accession: NP_001941

UniProt: Q16254

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, Regulation of Cell Size