KIF21A antibody (AA 551-600)

Catalog No. ABIN6736732

This Rabbit Polyclonal antibody specifically detects KIF21A in WB, IHC and IHC (p). It exhibits reactivity toward Human, Mouse, Dog, Horse, Cow, Guinea Pig, Rat and Monkey.

Quick Overview for KIF21A antibody (AA 551-600) (ABIN6736732)

Target: KIF21A (Kinesin Family Member 21A (KIF21A))

Reactivity: Human, Mouse, Dog, Horse, Cow, Guinea Pig, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KIF21A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-KIF21A Antibody

Binding Specificity: AA 551-600

Brand: IHC-plus™

Specificity: Human KIF21A

Predicted Reactivity: Percent identity by BLAST analysis: Human, Dog, Bovine (100%) Rabbit, Pig (92%) Mouse, Horse (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa551-600 of human KIF21A (Q7Z4S6, NP_060111). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Galago, Marmoset, Dog, Bovine (100%), Elephant, Bat, Rabbit, Pig (92%), Mouse, Horse, Opossum (85%), Rat (78%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for KIF21A

Target: KIF21A (Kinesin Family Member 21A (KIF21A))

Alternative Name: KIF21A

Background: Name/Gene ID: KIF21A

Synonyms: KIF21A, CFEOM3B, FEOM3A, FEOM1, KIAA1708, Kinesin family member 21A, Kinesin-like protein KIF2, Kinesin-like protein KIF21A, KIF2, CFEOM1

Gene ID: 55605

NCBI Accession: NP_060111

UniProt: Q7Z4S6

Pathways: M Phase