OLIG2 antibody (AA 236-285)

Catalog No. ABIN214131

This anti-OLIG2 antibody is a Rabbit Polyclonal antibody detecting OLIG2 in WB, IHC and IHC (p). Suitable for Human.

Quick Overview for OLIG2 antibody (AA 236-285) (ABIN214131)

Target: OLIG2 (Oligodendrocyte Lineage Transcription Factor 2 (OLIG2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This OLIG2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-OLIG2 Antibody

Binding Specificity: AA 236-285

Brand: IHC-plus™

Specificity: Human Olig2

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Mouse, Rat, Guinea pig (92%) Chicken (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa236-285 of human OLIG2 (Q13516, NP_005797). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Baboon, Monkey (100%), Galago, Marmoset, Mouse, Rat, Bat, Guinea pig, Armadillo (92%), Chicken (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: PBS buffer with 2 % sucrose

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8°C for up to 1 week. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for OLIG2

Target: OLIG2 (Oligodendrocyte Lineage Transcription Factor 2 (OLIG2))

Alternative Name: OLIG2

Background: Name/Gene ID: OLIG2

Synonyms: OLIG2, BHLHB1, BHLHe19, OLIGO2, PRKCBP2, RACK17

Gene ID: 10215

NCBI Accession: NP_005797

UniProt: Q13516