SIRT2 antibody (N-Term)

Catalog No. ABIN6736754

The Rabbit Polyclonal anti-SIRT2 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect SIRT2 in samples from Human, Monkey, Rabbit and Guinea Pig.

Quick Overview for SIRT2 antibody (N-Term) (ABIN6736754)

Target: SIRT2 (Sirtuin 2 (SIRT2))

Reactivity: Human, Monkey, Rabbit, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIRT2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SIRT2 Antibody

Binding Specificity: N-Term

Brand: IHC-plus™

Specificity: Human SIRT2

Predicted Reactivity: Percent identity by BLAST analysis: Human, Rabbit (100%) Mouse, Rat, Bovine, Horse, Pig (92%) Guinea pig (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human SIRT2 (Q8IXJ6, NP_036369). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Rabbit (100%), Mouse, Rat, Elephant, Bovine, Bat, Horse, Pig (92%), Galago, Guinea pig (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for SIRT2

Target: SIRT2 (Sirtuin 2 (SIRT2))

Alternative Name: SIRT2 / Sirtuin 2

Background: Name/Gene ID: SIRT2

Synonyms: SIRT2, SIR2, Sir2-related protein type 2, SIR2L, SIR2L2, Sirtuin 2, Silent information regulator 2, SIR2-like protein 2, Sirtuin type 2

Gene ID: 22933

NCBI Accession: NP_036369

UniProt: Q8IXJ6