SIRT7 antibody (AA 317-366)

Catalog No. ABIN214146

This anti-SIRT7 antibody is a Rabbit Polyclonal antibody detecting SIRT7 in WB, IHC and IHC (p). Suitable for Human, Rat, Mouse, Dog, Cow, Horse, Pig and Bat.

Quick Overview for SIRT7 antibody (AA 317-366) (ABIN214146)

Target: SIRT7 (Sirtuin 7 (SIRT7))

Reactivity: Human, Rat, Mouse, Dog, Cow, Horse, Pig, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIRT7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SIRT7 Antibody

Binding Specificity: AA 317-366

Specificity: Human SIRT7

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Horse, Pig (100%) Guinea pig (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa317-366 of human SIRT7 (Q9NRC8, NP_057622). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Mouse, Rat, Dog, Bovine, Bat, Horse, Pig (100%), Gibbon, Galago, Marmoset, Opossum, Guinea pig (92%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 50 μL sterile ddH2O.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for SIRT7

Target: SIRT7 (Sirtuin 7 (SIRT7))

Alternative Name: SIRT7 / Sirtuin 7

Background: Name/Gene ID: SIRT7

Synonyms: SIRT7, SIR2L7, Sirtuin type 7, SIR2-like protein 7, Sir2-related protein type 7, Sirtuin 7

Gene ID: 51547

NCBI Accession: NP_057622

UniProt: Q9NRC8