TCF4 antibody (AA 73-122)

Catalog No. ABIN6736759

The Rabbit Polyclonal anti-TCF4 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect TCF4 in samples from Human, Mouse, Rat, Horse, Pig, Dog, Monkey, Cow and Rabbit.

Quick Overview for TCF4 antibody (AA 73-122) (ABIN6736759)

Target: TCF4 (Transcription Factor 4 (TCF4))

Reactivity: Human, Mouse, Rat, Horse, Pig, Dog, Monkey, Cow, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TCF4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-TCF4 Antibody

Binding Specificity: AA 73-122

Brand: IHC-plus™

Specificity: Human TCF4

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Horse, Pig (100%) Guinea pig (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa73-122 of human TCF4 (P15884, NP_003190). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Rabbit, Horse, Pig, Opossum (100%), Guinea pig (92%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from 2 % Sucrose, PBS.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for TCF4

Target: TCF4 (Transcription Factor 4 (TCF4))

Alternative Name: TCF4

Background: Name/Gene ID: TCF4
Family: Transcription factor

Synonyms: TCF4, BHLHb19, E2-2, ITF-2, ITF2, PTHS, SEF2-1B, SEF2-1, SEF2-1A, SL3-3 enhancer factor 2, SEF2, SEF-2, TCF-4, Transcription factor 4

Gene ID: 6925

NCBI Accession: NP_003190

UniProt: P15884

Pathways: WNT Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Regulation of Hormone Metabolic Process, Carbohydrate Homeostasis, Stem Cell Maintenance, Protein targeting to Nucleus